LOCUS       pLV-[hsa-miR-597]        9151 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7072..7932
                     /label=Amp(R)
     promoter        8133..8673
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5677..5911
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6486..6941)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5983..6113
                     /label=SV40 pA
     misc_feature    6114..6327
                     /label=SV40 ori
     misc_feature    4998..5594
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3616
                     /label=intron
     CDS             3663..4988
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3406
                     /label=hsa-mir-597 precursor
BASE COUNT     2172 a      2369 c      2469 g      2141 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg tcttcacctt 
     3121 gcacagtctt tatagctgtc acagaggata aaggaaagtt aatttataaa ctaagtattt 
     3181 taccttttga tccccatcag ttaagatgtt acttactcta cgtgtgtgtc actcgatgac 
     3241 cactgtgaag acagtaaaat gtacagtggt tctcttgtgg ctcaagcgta atgtagagta 
     3301 ctggtcgacc ttgtctaact tcacgttccc ttgaggctga cttgctgtca cctgaagaga 
     3361 ggtgtaatgc aatcggtctc ctgaccggaa gtccactctt tcatttgcta gctcgagctt 
     3421 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac 
     3481 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt 
     3541 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt 
     3601 ttttcttcca tttcaggtgt cgtgaaaact acccctctag agtcgagcta ccggtcgcca 
     3661 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca 
     3721 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg 
     3781 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc 
     3841 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc 
     3901 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc 
     3961 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg 
     4021 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg 
     4081 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg 
     4141 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg 
     4201 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg 
     4261 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 
     4321 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag gacccaccgg 
     4381 tcgccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca 
     4441 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 
     4501 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 
     4561 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 
     4621 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 
     4681 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 
     4741 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 
     4801 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 
     4861 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 
     4921 tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg 
     4981 gtgcctgagc ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac 
     5041 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 
     5101 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 
     5161 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 
     5221 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 
     5281 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 
     5341 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 
     5401 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 
     5461 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 
     5521 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 
     5581 cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta caaggcagct 
     5641 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 
     5701 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 
     5761 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 
     5821 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 
     5881 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 
     5941 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 
     6001 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     6061 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 
     6121 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     6181 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 
     6241 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc 
     6301 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 
     6361 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 
     6421 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 
     6481 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 
     6541 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 
     6601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 
     6661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 
     6721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 
     6781 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 
     6841 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 
     6901 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 
     6961 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     7021 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 
     7081 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 
     7141 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 
     7201 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 
     7261 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 
     7321 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 
     7381 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 
     7441 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 
     7501 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 
     7561 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 
     7621 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 
     7681 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 
     7741 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 
     7801 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 
     7861 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 
     7921 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 
     7981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     8041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     8101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     8161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     8221 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 
     8281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     8341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     8401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     8461 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     8521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     8581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     8641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     8701 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     8761 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 
     8821 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 
     8881 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 
     8941 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 
     9001 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 
     9061 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac 
     9121 cctcactaaa gggaacaaaa gctggagctg c 
//
